Journal: The Canadian journal of neurological sciences. Le journal canadien des sciences neurologiques

Article Title: Utility of Repeat Endpoint Quaking-Induced Conversion Testing in Creutzfeldt-Jakob Disease.

doi: 10.1017/cjn.2022.306

Figure Lengend Snippet: Figure 2: Neuropathologic findings typical of Creutzfeldt–Jakob disease. Samples from the left temporal neocortex reveal patchy spongi- form change with select vacuoles abutting cort- ical neurons (A, arrowheads) (hematoxylin and eosin, scale bar = 50 um). Immunoperoxidase preparations confirm the presence of fine punc- tate and larger granular collections of abnormal prion proteins (B) (anti-PrPSc, ECM Biosciences, Versailles, Kentucky, scale bar = 50 um).

Article Snippet: Immunoperoxidase preparations confirm the presence of fine punctate and larger granular collections of abnormal prion proteins (B) (anti-PrPSc, ECM Biosciences, Versailles, Kentucky, scale bar= 50 um).

Techniques:

Journal: The Journal of Cell Biology

Article Title: The Rac activator Tiam1 is required for α3β1-mediated laminin-5 deposition, cell spreading, and cell migration

doi: 10.1083/jcb.200509172

Figure Lengend Snippet: Adhesion and signaling of WT and Tiam1−/− keratinocytes. (A) Keratinocytes were seeded on an exogenous LN5 or LN1 matrix. After 30 min, the number of adherent cells was quantified in an enzymatic assay. Phase-contrast images were taken before cell lysis. The values in the histogram are means ± SD. Note that both WT and Tiam1−/− keratinocytes hardly adhere to and spread on LN1. Bar, 50 μm. (B) Growth factor–starved WT and Tiam1−/− keratinocytes were detached from the culture dish and reseeded on a LN5-coated surface in growth factor–free medium. A sample of the cells in suspension was lysed and the attached cells were lysed after 10, 30, 60, and 180 min. Lysates were immunoblotted for phosphoY397-FAK. β-Actin was used as a loading control. (C) Cells seeded as described in B were lysed after 5, 15, and 45 min. Lysates were immunoblotted for phospho-Src, -Stat3, and -ERK1/2. β-Actin was used as a loading control.

Article Snippet: All ECM proteins except LN5 were coated to culturing dishes overnight at 4°C at the following concentrations: 10 μg/ml FN (isolated from human plasma); 10 μg/ml LN1 (Becton Dickinson); 10 μg/ml VN (Sigma-Aldrich); 20 μg/ml Col I (Vitrogen/Nutacon); and 25 μg/ml Col IV (Becton Dickinson).

Techniques: Enzymatic Assay, Lysis, Suspension, Control